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Image Search Results
Journal: Advanced Science
Article Title: Single‐Cell Atlas of Human Ovaries Reveals The Role Of The Pyroptotic Macrophage in Ovarian Aging
doi: 10.1002/advs.202305175
Figure Lengend Snippet: Age‐related changes in macrophages and subsequent impacts on ovarian microenvironment A) Immunostaining of CD14 in young (upper panel) and middle‐aged (lower panel) ovarian sections. Bar:100um. The number of CD14 positive cells was counted and displayed in the right statistical graph. B) Proportions of TRM and MDM subsets in the total macrophages in young and middle‐aged women. C) Pseudotime visualization of the distributions of macrophage populations in young and middle‐aged women. Each dot represents one cell. D) Bar plot illustrating the numbers and proportions of each macrophage subset in the young and middle‐aged groups. E) Boxplot diagram showing the relative expression of CASP1 , NLRP3 and IL1B in each macrophage subcluster from young and middle‐aged women. F) Boxplot diagram showing the scores of pyroptosis pathway activity in each macrophage cluster from the two groups. G) Bubble plots showing the mean strength of selected ligand‐receptor pairs in macrophage subclusters with other cell types. The dot size represents the p value. H) Boxplots plots showing the different expression of selected ligands in macrophage subclusters between the young and middle‐aged groups. I) Representative images of senescence‐associated β‐galactosidase (SA‐beta‐gal) staining of stromal cells treated with SPP1 and EGF. Statistical analyses of SA‐beta‐gal‐positive cell rates were shown in the right image.
Article Snippet: SA‐beta‐gal staining was performed on primary human ovarian stromal cells (iCell Bioscience Inc, HUM‐iCell‐f001) using
Techniques: Immunostaining, Expressing, Activity Assay, Staining
Journal: Advanced Science
Article Title: Single‐Cell Atlas of Human Ovaries Reveals The Role Of The Pyroptotic Macrophage in Ovarian Aging
doi: 10.1002/advs.202305175
Figure Lengend Snippet: Pyroptotic macrophages promote stromal cell senescence. A) Representative images of senescence‐associated β‐galactosidase (SA‐beta‐gal) staining of stromal cells treated with the supernatant of TNF‐α/IFN‐γ‐stimulated THP1 cells, the supernatant of LPS/Nig‐stimulated THP1 cells or the supernatant of unstimulated THP1 cells (scale bar, 500 µm). Statistical analyses of SA‐beta‐gal‐positive cell rates were shown in the right image. B) The relative expression of fibrosis‐related genes in ovarian stromal cells treated with supernatant of unstimulated or stimulated THP1 cells. The results were analyzed by ANOVA. C) Representative IHC staining for GSDMD showing the elevated expression of GSDMD in the middle‐aged group (scale bar, 100 µm). The insert at top left corner of each image is the magnified image (scale bar, 250 µm). D) EREG concentration in follicular fluids measured by ELISA. E) Flow cytometric analysis of follicular fluids from IVF patients: representative flow cytometric dot plots of macrophages in follicular fluids from each group. Statistical graph of the percentages of macrophages in follicular fluids. F) Representative histogram plots and the statistical graph of the MFI of GSDMD. MFI: mean fluorescence intensity.
Article Snippet: SA‐beta‐gal staining was performed on primary human ovarian stromal cells (iCell Bioscience Inc, HUM‐iCell‐f001) using
Techniques: Staining, Expressing, Immunohistochemistry, Concentration Assay, Enzyme-linked Immunosorbent Assay, Fluorescence